Gatterdam, Volker

Research Interests

• photolithography
• chemical synthesis
• wave-guide biosensors
• label-free detection methods

Focal molography is a new label-free detection method, which will allow the real time investigation of complex molecular interactions in the presence of a biological environment [1], [2]. Thereby molography opens up new perspectives and possibilities in diagnostics but also for pharmaceutical relevant protein interaction studies. This new detection principle is based on the scattering of coherent light on also coherently assembled molecules. This specific arrangement of molecules is called a mologram. The scattered light depends on the mass and refractive index of the coherent pattern, while the optimized arrangement and shape of the lines evoke a holographic focusing of the wave field into a focal point of an Airy disk. Background scattering by randomly attached molecules can be easily subtracted from the intensity of the Airy disk.

[1] C. Fattinger, “Focal Molography: Coherent Microscopic Detection of Biomolecular Interaction,”
Physical Review X 4, 031024 (2014) – open access.
[2] G. Binnig, “Coherent Signal Picks Out Biomolecular Interactions,” Physics 7, 84 (2014) – open access.

Publications

05/2014 - P. Arnold, T.-M. Decker, J. Markl, V. Gatterdam, R. Tampé, Patricia Himmels, S. Weiß, P. Bartholomäus, U. Dietrich, R. Dürr, Antigenic and 3D Structural Characterization of Soluble X4 HIV-1 Env Trimers and an R5 V3 Exchange Mutant. Retrovirology 11, 42 (2014).

04/2014 - V. Gatterdam, R. Ramadass, T. Stoess, J. Wachtveitl, A. Heckel, R. 
Tampé, Three-Dimensional Protein Networks Assembled by Two-Photon Activation. Angew. Chem. Int. Ed. Engl. 22, 5680–5684 (2014); Dreidimensionale Proteinnetzwerke durch Zwei-Photonen - Aktivierung Angew. Chem 22, 5787–5791 (2014)

09/2012 - O. Rossier, V. Octeau, J.-B. Sibarita, C. Leduc, B. Tessier, D. Nair, V. Gatterdam, O. Destaing, C. Albigès-Rizo, R. Tampé, L. Cognet, D. Choquet, B. Lounis, and G. Giannone, Integrins β1 and β2 Exhibit Distinct Dynamic Nanoscale Organizations Inside Focal Adhesions. Nat Cell Biol 14, 1057–1067 (2012).

09/2012 - J. Hartmann, T. V. Tran, J. Kaudeer, K. Oberle, J. Herrmann, I. Quagliano, T. Abel, A. Cohnen, V. Gatterdam, A. Jacobs, B. Wollscheid, R. Tampé, C. Watzl, A. Diefenbach, and J. Koch, The Stalk Domain and the Glycosylation Status of the Activating Natural Killer Cell Receptor NKp30 are Important for Ligand Binding. J Biol Chem 287, 31527–31539 (2012).

03/2012 - V. Gatterdam*, T. Stoess*, C. Menge, A. Heckel, R. Tampé, Caged Glutathione - Triggering Protein Interaction by Light. Angew Chem Int Ed 51, 3960–3963, (2012); Photoaktivierbares Glutathion - lichtgesteuerte Proteinwechselwirkung. Angew Chem 124, 4027–4030 (2012).

03/2012 - R. Wei, V. Gatterdam, R. Wieneke, R. Tampé, U. Rant, Stochastic Sensing of Proteins with Receptor-Modified Solid-State Nanopores. Nat Nanotechnol 7, 257–263 (2012).

Conferences, Talks & Courses

2015/03 - Label-free Technologies; Cambridge, MA, USA (Oral presentation)
2014/11 - CCMX Advanced Course (Combining Structural & Analytical Investigations of Matter at the Micro-, Nano- and Atomic Scale), Zürich, Switzerland
2014/10 - 5th Roche Postdoctoral Fellow Symposium, Munich, Germany (Poster)
2013/01 - Evening lecture "FIRST" students, Frankfurt, Germany (Oral presentation)
2012/10 - GBM “Studiengruppe Redoxbiologie”, Heidelberg, Germany (Oral presentation)
2012/11 - Label-Free Technologies: Advances and Applications, Amsterdam, Netherlands
2012/11 - 2nd European ProteOnTM User Meeting von Bio-Rad, Amsterdam, Netherlands
2011/09 - Molecular Life Sciences 2011, Frankfurt, Germany
2010/11 - 1st MicroCal User Meeting von GE Healthcare, Hamburg, Germany