Delange, Victoria, Dr.
ETH Zürich
PhD Thesis 2015 / Master Thesis 2010
Research Interests
Keeping it simple is ironically not that easy! Protein microarray technology is a field dominated by robotics and lithography, which are expensive and complex techniques. Our goal is to make high tech sensing accessible and to improve protein microarrays in the process.
My research focuses on developing the FoRe microarray, which combines forward and reverse phrase arrays into a novel 3D design. By combining the traditional microarray approaches we can exploit the advantages of each and eliminate several drawbacks (i.e. protein loss, large sample volumes, and cross reactivity between detection antibodies). The FoRe array is capable of multiplexed analysis of several μl-sized samples in parallel. We demonstrated the device with fluorescent immunoassays and are now focused on expanding its applications.
Fig. 1 A) Capture antibodies are passively adsorbed to the wax-patterned nitrocellulose. B) Functionalised nitrocellulose arrays are stacked to create an array of multiplexed affinity columns. Ab1−Ab4 refers to four different capture antibodies. C) The layers of nitrocellulose are assembled in a device to aid alignment and sample injection. The cut away side view shows one row of five channels (samples 1−5). D) The stack is disassembled and the layers are incubated in a specific detection antibody (dAb). The 2D arrays are imaged with standard fluorescence-based readout.
Education
- ETH Zurich, Switzerland 2008 to 2010
Masters in Biomedical Engineering - University of Victoria, Victoria, British Columbia 2003 to 2008
Bachelor of Electrical Engineering with distinction, specializing in Electronics.
Publications
- Twist on Protein Microarrays: Layering Wax-Patterned Nitrocellulose to Create Customizable and Separable Arrays of Multiplexed Affinity Columns
V. de Lange and J. Vörös
Anal. Chem., DOI: 10.1021/ac501211m - Microarrays Made Easy: Biofunctionalized Hydrogel Channels for Rapid Protein Microarray Production
V. de Lange, A. Binkert, J. Vörös, and M. Bally
Appl. Mater. Interfaces, vol. 3, no. 1, pp. 50-57m 2011. - Inside Vertical-Cavity Surface-Emitting Lasers: Extracting the Refractive Index From Spatial-Spectral Mode Images
V. de Lange, K. Sun, and R. Gordon
IEEE J. Quantum Electron., vol. 43, no. 3, pp. 225-229, Mar. 2007. - Vesicles for Signal Amplification in a Biosensor for the Detection of Low Antigen Concentrations
D.Grieshaber , V. de Lange , T. Hirt, Z. Lu, and J. Vörös
Sensors, vol. 8, pp. 7894-7903, Dec. 2008. - On-Chip Surface-Based Detection with Nanohole Arrays
A. De Leebeeck, L. K. S. Kumar, V. de Lange, D. Sinton, R. Gordon, and A. Brolo
Anal. Chem., vol. 79, pp 4094–4100, 2007.
Conferences
- "The FoRe Microarray: 3D Multiplexing in Clinical Samples," 4th Global Reverse Phase Protein Array Workshop, Paris, October 24-25, 2014. Invited talk.
- V. de Lange, J. O. Sass, J. Vörös, "The FoRe Microarray: Multiplexed Protein Detection in Clinical Sample Volumes," Swiss Society for Biomedical Engineering Annual Meeting, ETH Zurich, August 2014. Poster presentation.
This poster won the SSBE poster prize - V. de Lange and J. Vörös, "The FoRe Microarray: A Simple and Inexpensive Technique for Multiplexed Protein Detection in Minute Clinical Samples," MaP Graduate Symposium & MaP Award Ceremony 2014, Zurich, Switzerland, June 2014. Poster presentation.
This poster won the MaP Poster Prize - V. de Lange and J. Vörös, "The FoRe Microarray: A Layered Vertical Flow Device for Multiplexed Protein Detection in Clinical Sample Volumes," Flow14 international Conference on micro- and nanofluidics , University of Twente, Netherlands, May 2014. Poster presentation.
This poster was nominated for the poster prize - V. de Lange and J. Vörös, "In Forward and Reverse: Combining two Microarray Styles to Pattern Proteins in Three-Dimensions," Biological Surfaces and Interfaces, San Feliu, Spain, June 2013. Poster presentation.
- V. de Lange and J. Vörös, “Patterning Microarrays in all Three Dimensions,” 2012 MRS Fall Meeting & Exhibit and 2012 Workshop on Capillary-based Microfluids for Bioanalysis, Boston, USA, Nov. 2012. Poster presentation.
- V. de Lange, J. Voros, “Microarrays in Three Dimensions,” NanoBioTech, Montreux, Switzerland, Nov. 2012. Poster presentation.
This poster won the BioAlps prize for the best poster. - V. de Lange and J. Vörös, “Fast and Easy Biochip Fabrication: Multiple Copies from Functionalized Hydrogel Channels,” Advances in Biodetection and Biosensors, Edinburgh, Scotland, March 2012. Poster presentation.
- V. de Lange, A. Binkert, M. Bally, J. Vörös, “A Simple Approach to Array Fabrication: Producing Multiple Biochips Copies from Functionalized Hydrogel Channels,” Biological Surfaces and Interfaces - ESF-EMBO Symposium, Sant Feliu de Guixols, Spain, June 2011.
- V.de Lange, K.Sun, and R. Gordon, “Nondestructive Internal Device Characterization of an Oxide-Confined Vertical-Cavity Surface-Emitting Laser,” Conf. on Lasers and Electro-Optics/Quantum Electronics and Laser Science Conf., Baltimore, USA, 2007. Poster presentation
Research Internships
- Laboratory of Biosensors and Bioelectronics, ETH Zürich, Switzerland
Research Assistant, Coop Work Term September 2007 to December 2007 - Airway Mechanics Laboratory, Dalhousie University Halifax, Nova Scotia
Research Assistant, Coop Work Term January 2007 to August 2007 - Optics Laboratory, University of Victoria Victoria, British Columbia
Research Assistant, Coop Work Term June 2006 to August 2006