Dorwling-Carter, Livie
Livie Dorwling-Carter
PhD student, August 2014 to December 2018
Research Interest : Cell electrophysiology on the Fluid Force Microscope (FluidFM)
Scanning ion conductance microscopy (SICM) and patch-clamp are two powerful techniques allowing to study the cell electrical properties by relying on a similar electrical setup able to measure picoampere ionic currents flowing through a tip aperture. While SICM allows to image soft structures such as cells in near-physiological conditions without mechanical contact external page (Hansma et al., 1989), the patch-clamp (external page Neher and Sakmann, 1976) has become the gold standard for measuring the activity of ion channels, which are transmembrane pore-forming proteins involved in the cell electrical activity by a controlled flow of ions through those pores.
By combining the SICM and patch-clamp on the FluidFM as depicted underneath, the two techniques get their possibilities expanded thanks to the additional force feedback of the FluidFM, especially powerful for the gentleness of the patching or the scanning. With such an additional force control, we were able to patch beating cardiac cells (external page Ossola et al., 2015) whereas the SICM could benefit from its combination with an AFM to reduce tip/sample interactions (external page Dorwling-Carter et al., 2015).
Education
- Msc Biomedical Engeering, ETH Zurich
- Dipl.El.Ing-French engineering School (SUPELEC)
Posters
Towards nano-mimicking the cell bilayer architecture for spontaenous probe insertion and gigaseal formation in force-controlled patch clamp - L.Dorwling-Carter, M. Aramesh, J. Vörös, T. Zambelli -FEBS Workshop-Biological Surfaces and Interfaces: Interface Dynamics, 2-7 July 2017, Sant Feliu de Guixols, Catalonia, Spain.
Force-controlled electrophysiology: achievements and optimization
L.Dorwling-Carter, D.Ossola, J.Vörös, H.Abriel, T.Zambelli
7th International Workshop on Cardiac Mechano-Electric Coupling and Arrhythmias, 21-24 September 2016, Freiburg, Germany
This poster won the Honourable Mention at the Trainee Poster Competition
Optimizing the seal interface of the probe and cell for force-controlled patch-clamp
L.Dorwling-Carter, D.Ossola, J.Vörös, H.Abriel, T.Zambelli
Biointerfaces International 2016, 23-25 August 2016, Zürich, Switzerland
Seal optimization for force-controlled patch-clamp
L.Dorwling-Carter, D.Ossola, J.Vörös, H.Abriel, T.Zambelli
Nano Bio Tech, 16-18 November 2015, Montreux, Switzerland
Scanning ion conductance microscopy with simultaneous force recording
L.Dorwling-Carter, D.Ossola, J.Vörös, T.Zambelli
MicroTas 2015 , 25-29th October 2015, Gyeongju, Korea
Force-controlled electrophysiology
L.Dorwling-Carter, D.Ossola, M.Y.Amarouch, P.Behr, J.Vörös, H.Abriel, T.Zambelli
8th International Ascona Workshop on Cardiomyocyte Biology, 3-7th May 2015, Ascona, Switzerland
This poster won the Award for Best Contribution.
Non-contact imaging combining the scanning ion conductance microscopy with the atomic force microscope
L. Dorwling-Carter, D. Ossola, J. Vörös, T. Zambelli
6th AFM BioMed Conference , 13-17 December 2014, San Diego, CA, USA
Presentations
The Fluidic Force Microscope: a versatile tool for force-controlled electrophysiology
L.Dorwling-Carter, J.Vörös, T.Zambelli
NANOinBIO, Advances for Life & Materials Sciences, 31 May-5th June 2016, Le Gosier, Guadeloupe
Force-controlled Patch Clamp and Scanning Ion Conductance Microscopy with the FluidFM
L.Dorwling-Carter, J. Vörös, T. Zambelli
7th AFM BioMed Conference, 11-15 April 2016, Porto, Portugal
This presentation won the best oral communication award.
The FluidFM for force-controlled patch-clamp and scanning ion conductance microscopy
L.Dorwling-Carter, D.Ossola, J.Vörös, T.Zambelli
4th FluidFM User Meeting, 2nd June 2015, Zürich, Switzerland